Phospholipidosis assay

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August undefined, 2024

WebIn this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H 2 … WebPhospholipidosis is a lysosomal storage disorder characterized by the excess accumulation of phospholipids in tissues, often stimulated by drugs. Drug induced hepatotoxicity is a …

Cell-Based Imaging Assay for Detection of Phospholipidosis

WebJun 29, 2012 · The presence or absence of phospholipidosis in the in vitro assay correlated well with ultrastructural findings and drug accumulation in rat tissues. ABT-770, which produced phospholipidosis ... WebJul 2, 2008 · In vitro screening assays were developed in HepG2 cells, rat primary hepatocytes, and rhesus monkey hepatocytes using the fluorescent-labeled phospholipid probe N - (7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl- sn -glycero-3-phosphoethanolamine (NBD-PE) or Nile Red lipid stain. grace bible church elmhurst youtube

Modulation of in Vitro SARS-CoV-2 Infection by Stephania

WebTraductions en contexte de "monitored by assay" en anglais-français avec Reverso Context : If self-injection with Improvac is suspected, reproductive physiology should be monitored by assay of testosterone or oestrogen levels (as appropriate). The risk WebAug 2010 - Oct 20144 years 3 months. Jalandhar,Punjab,India. The active area of research at University was Metabolic disorder (Diabetes).I was involved in in-vitro selection & characterization, target validation for metabolic targets, assay development and condition optimization, its high throughput screening, selection of hits among phyto ... WebFeb 1, 2007 · Phospholipidosis was determined by algorithms that evaluated the granular texture of the perinuclear area as an indicator of the amount of phospholipidosis vacuoles. ... High-content screening... chili\u0027s midlothian va

Detection of Phospholipidosis Induction: A Cell-Based Assay in …

WebJun 22, 2024 · To investigate the role of phospholipidosis in antiviral activity in vitro, we tested 19 drugs for their induction of this effect in A549 cells using the well-established … WebPhospholipidosis (PLD) is a lysosomal storage disorder induced by compounds, notably cationic amphiphilic drugs, which although reversible interferes with cellular … chili\\u0027s middletown riWebMar 5, 2015 · Synthesis and phospholipidosis effect of a series of cationic amphiphilic compounds: a case study to evaluate in silico and in vitro assays Article Full-text available chili\u0027s middletown ri menu

"WebIn this study, a cell-based phospholipidosis assay has been developed using the LipidTOX Red reagent in HepG2 cells and miniaturized into a 1536-well plate format. To validate this assay for high-throughput screening (HTS), the LOPAC library of 1280 compounds was screened using a quantitative HTS platform. A group of known phospholipidosis ... " - Phospholipidosis assay

Phospholipidosis assay

WebSep 3, 2013 · In this study, a cell-based phospholipidosis assay has been developed using the LipidTOX Red reagent in HepG2 cells and miniaturized into a 1536-well plate format. To validate this assay for high ... WebFluorescent images were captured and analyzed using the Discovery-1 automated cellular imaging system. Eleven out of the 12 selected positive compounds and all negative compounds were properly assigned as positive and negative inducers of phospholipidosis, respectively, indicating the high degree of sensitivity and specificity of this assay.

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WebCyprotex offer a number of other services including reactive metabolite detection using trapping agents, a cell stress panel, drug-induced phospholipidosis and steatosis, lysosomal trapping, hemolysis, mitochondrial toxicity (mitochondrial biogenesis assay, Glu/Gal assay, functional mitochondrial toxicity assay and the mitochondrial respiratory ... WebWhen studying phospholipidosis/steatosis in vitro, IONTOX uses HepG2 cells in combination with LipidTox Red, as our primary model to discriminate between phospholipidosis …

WebTo identify compounds that induce intracellular phospholipidosis, we used a high-content imaging method to optimize and validate the cell-based PLD assay. The assay was … WebPhospholipidosis (PL) is a phospholipid storage disorder, resulting in excessive accumulation of phospholipids in lysosomes of various tissue types [199, 200]. From:Progress in Medicinal Chemistry, 2011 Related terms: Amiodarone Patient Phospholipid Storage Disease Lysosome Storage Disease Niemann Pick Disease …

WebJun 21, 2024 · 1. Introduction. Drug-induced phospholipidosis (DIPLD) is characterized by excessive intracellular accumulation of phospholipids as a result of drug treatments [1].Since the first DIPLD case reported in 1948 [2], an increasing number of drugs were identified as PLD inducers, including antibiotics, antidepressants, antipsychotics and so … WebThe LipidTOX assay series includes neutral lipid stains for fixed cells and phospholipid stains for live cells, which can be combined in multiplexed assays. Automatically …

WebSuch assays rely heavily on the development prospective assays aim to predict toxicities that could lead to the ter- of human physiologically relevant model systems in combination with mination of a drug candidate or an entire development programme, appropriate toxicity end points. and bespoke retrospective assays are used for issue resolution ...

WebinstaCELL® Phospholipidosis Assay Kits. Drug induced accumulation of phospholipids (phospholipidosis) is associated with clinical toxicities. Cell‐based assays can help to … chili\\u0027s midland txWebLC-MS lipid profiling of HepG2 cells is proposed as an in vitro assay to study and anticipate phospholipidosis and steatosis. Cells with and without preincubation with a mixture of … grace bible church fort worth texasWebJun 12, 2007 · Using an expanded set of compounds, we show that this assay correctly identified 100% of PLD-positive and -negative compounds. Dose-dependent increases in intracellular fluorescent phospholipid accumulation were observed. We found that this assay was less time consuming, more sensitive, and higher throughput than gene expression … grace bible churches near meWebcontent phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) plat- form. This assay has been optimized and validated in HepG2 and HepRG cells, and miniaturized into a grace bible church eventsWebMar 1, 2024 · The EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large … grace bible church garden city ksWebMar 1, 2024 · This assay has been optimized and validated in HepG2 and HepRG cells, and miniaturized into a 1536-well plate, thus can be used for high-throughput screening (HTS) to identify chemical compounds... grace bible church faribault mnWeb+ selectively discriminates phospholipidosis from steatosis assay ready HepG2 cells assay ready HepG2 cells have an instantly high viability, low debris and attach within 24 hours. drug induced phospholipidosis assay ready HepG2 were treated for 48 hours with Sertraline, a potent inductor of phospholi-pidosis, and Cyclosporine A for negative ... chili\u0027s midland tx