Fluoresceinated peptides
WebJan 16, 2007 · The helical content of each peptide was calculated as reported previously.3 E. Ex vivo Protease Stability The fluoresceinated peptides (2.5 µg) were incubated with fresh mouse serum (20 µL) at 37°C for 0-24 hours. The level of intact fluoresceinated compound was determined by flash freezing the serum WebAntifluorescein antibody molecules were used to interrupt the stimulation of neutrophils by a fluoresceinated chemotactic peptide. From the results... Fluoresceinated chemotactic …
Fluoresceinated peptides
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WebThe development of methods to transport peptides into cells via a passive mechanism would greatly aid in the development of therapeutic agents. We recently demonstrated … WebWe recently demonstrated that an impermeable fluoresceinated pentapeptide enters the cytoplasm and nucleus of COS 7 cells in the presence of a host- [2]rotaxane by a mechanism that does not...
WebBinding of fluoresceinated PEDF to retina cryosections was detected by confocal microscopy. Results: Radioligand-binding assays showed that (125)I-PEDF bound in a specific and saturable fashion to one class of sites on retina membranes (K (d) = 2.5-6.5 nM; maximum binding [B (max)] = 1-48 x 10 (10) sites/retina). WebMay 7, 2002 · This peptide sequence was modified further on resin to incorporate donor/quencher pairs to generate substrates for use in fluorescence resonance energy …
WebJul 8, 2004 · Peptide nucleic acid (PNA) oligomers were conjugated to cell-penetrating peptides: pAnt, a 17-residue fragment of the Drosophila protein Antennapedia, and … WebWe recently demonstrated that an impermeable fluoresceinated pentapeptide enters the cytoplasm and nucleus of COS 7 cells in the presence of a host-[2]rotaxane by a mechanism that does not depend on an active cell-mediated process.
WebDec 1, 2002 · The synthetic peptides were labeled using a fluorescein amine (FS) labeling kit (Panvera, Madison, WI). The labeling reagent is a succinimidyl ester of fluorescein, which selectively forms an amide bond with N-terminal amino group of peptides or proteins at neutral pH; this reagent reacts more rapidly and forms a more stable derivative than FITC.
WebPeptides were synthesized by standard Fmoc solid-phase peptide synthesis on either an automated Tribute peptide synthesizer (Gyros Protein Technologies) or an automated … simon schama the history of nowWebJun 20, 1990 · In this study, several methods for controlled labelling of synthetic peptides by the use of fluorescent compounds (fluorescein isothiocyanate and dimethylaminonaphthalene sulfonyl chloride) were investigated. The first reagent yielded … simon schama power of art davidWebAug 12, 2024 · The fluoroalkylated peptides such as F-P1 show robust efficiency in cytosolic delivery of peptides into various cell lines such as 293T, NIH3T3, MCF7, and RAW264.7 cells ( Fig. 4F ). The high … simon schama truckload of troubleWebMar 20, 2006 · Titration of fluoresceinated, 500 kDa dextramers on CTL clones specific for Influenza- and Melan-A-derived peptides. The fluoresceinated, 500 kDa dextran was saturated with biotinylated HLA-A2 monomers loaded either with an Influenza-derived peptide (FLU, open circles) or with a melanoma-derived peptide (ELA, full circles). simon schama\\u0027s daughter chloe frances schamaWebFollowing fractionation, fluoresceinated peptides were immunoprecipitated using mAb 4-4-20 (anti-fluorescein) and immunoprecipitates were analysed on SDS–PAGE. To … simon schama of artWebWe have compared the kinetics of the responses of neutrophils to the kinetics of ligand-receptor interaction and internalization, using as a model ligand the fluoresceinated hexapeptide N-CHO-Nle-Leu-Phe-Nle-Tyr-Lys-Fluorescein (Nle, norleucine). Cellular responses, ie, membrane depolarization, enzy … simon schama on twitterWebpeptide fluorescence and the cellular response under kinetic spectroscopic conditions, we are able to probe a number of as-pects of neutrophil response to this peptide. Part of … simon schama the embarrassment of riches